Chemotaxis and phagocytosis of the neutrophils mobilized by granulocyte colony-stimulating factor in healthy donors for granulocyte transfusions

Potentiation of neutrophil functions with administration of granulocyte colony-stimulating factor to donors for granulocyte transfusions.

RAPID COMMUNICATION Effective Stimulation of Donors for Granulocyte Transfusions With Recombinant Methionyl Granulocyte Colony-Stimulating Factor

Effective granulocyte transfusion (GT) therapy has been hampered by the low yield of neutrophil granulocytes (PMN) obtainable from normal donors even by use of corticosteroid prestimulation, hydroxyethyl starch (HES), and modern leukapheresis (LA) techniques. To increase the PMN yield we performed LA in 22 healthy volunteer donors after a single subcutaneous administration of 300 fig of granulo...

Effective stimulation of donors for granulocyte transfusions with recombinant methionyl granulocyte colony-stimulating factor.

Effective granulocyte transfusion (GT) therapy has been hampered by the low yield of neutrophil granulocytes (PMN) obtainable from normal donors even by use of corticosteroid prestimulation, hydroxyethyl starch (HES), and modern leukapheresis (LA) techniques. To increase the PMN yield we performed LA in 22 healthy volunteer donors after a single subcutaneous administration of 300 micrograms of ...

Granulocyte transfusions from granulocyte colony-stimulating factor-treated donors: also a question of cell survival?

Recent articles published in this Journal'.* and one Editorial' focused on granulocyte (PMN) transfusions from donors pretreated with granulocyte colony-stimulating factor (G-CSF) to increase cell numbers. Although the therapeutical value of granulocyte transfusion in infected neutropenic patients is not definitively established, as discussed in the Editorial by Strauss,' the results reported s...

Cycling status of CD34+ cells mobilized into peripheral blood of healthy donors by recombinant human granulocyte colony-stimulating factor.

In this study, we assessed the functional and kinetic characteristics of highly purified hematopoietic CD34+ cells from the apheresis products of 16 normal donors undergoing glycosylated granulocyte colony-stimulating factor (G-CSF) treatment for peripheral blood stem cells (PBSC) mobilization and transplantation in allogeneic recipients. Mobilized CD34+ cells were evaluated for their colony-fo...